[The influence of AEDG and KE peptides on mitochondries stain and L7A ribosomes protein expression during human pineal gland and thymus cell senescence in vitro.].
Ivko. O M OM; Drobintseva. A O AO; Leont'eva. D O DO; Kvetnoy. I M IM; Polyakova. V O VO; Linkova. N S NS
Key Findings
- Epitalon increased the intensity of MitoTracker Red staining by about 1.5‑fold, indicating improved mitochondrial activity in aging pineal cells.
- Epitalon reduced the expression of ribosomal protein L7A by roughly 22% in pineal cell cultures, counteracting a senescence‑related increase.
- Similar but slightly weaker effects were seen with the KE peptide in thymic cells (15% reduction in L7A).
Practical Outcomes
- The data hint that epitalon might support mitochondrial function in pineal and thymic tissues, but the work is limited to cell cultures with no human dosing information. For biohackers, it reinforces existing interest in epitalon as a geroprotector but does not provide a new protocol or dosage to apply directly.
Summary
A lab study on human pineal and thymus cells found that the peptide epitalon (called AEDG) can boost markers of mitochondrial health and lower a ribosomal protein that rises when cells age, suggesting it may help keep these cells healthier in a dish.
Abstract
It was verified new molecular targets of geroprotective activity of AEDG (epitalon) and KE (vilon) peptides by the method of confocal laser scanning microscopy. It was shown that the MitoTracker Red mitochondries staining decreased and L7A ribosomal protein synthesis compensatory increased during pineal and thymic cell senescence in vitro. AEDG peptide increases in 1,5 times the square of MitoTracker Red mitochondries staining and decreases on 22% the expression of ribosomal protein L7A in cultures of human pineal gland cells during its senescence. KE peptide increases in 1,5 times the square of MitoTracker Red mitochondries staining and decreases on 15% the expression of ribosomal protein L7A in cultures of human thymic cells during its senescence. The square of MitoTracker Red mitochondries staining decreases and the expression of L7A ribosomal protein compensatory increases during pineal gland and thymic cells senescence. We can suppose that AEDG and KE peptides have a tissue-specific effect that normalizes the functions of mitochondria and ribosomes of pinealocytes and thymocytes.
Study Information
pubmed
2020