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GHRP-6

Growth Hormone Releasing Peptide-6, Growth hormone-releasing hexapeptide, His-D-Trp-Ala-Trp-D-Phe-Lys-NH2

A synthetic hexapeptide that stimulates growth hormone secretion by mimicking ghrelin and binding to GHS receptors in the pituitary gland.

Quick Stats
Studies 702
Trials 0
Formula C46H56N12O6
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Utility 4
pubmed Nov 1, 1996

Growth hormone (GH)-releasing peptide and GH releasing hormone stimulate GH release from subpopulations of somatotrophs in rats.

Mitani. M M; Kaji. H H; Abe. H H; Chihara. K K

In rats, the peptide GHRP-6 directly makes the pituitary gland release more growth hormone, and it works together with the natural hormone GHRH to give an even bigger boost, although the two just add up rather than multiply each other's effects. Different groups of hormone‑producing cells respond to GHRP-6, GHRH, or both, showing that the peptide has its own pathway for triggering growth hormone.

Utility 4
pubmed 1995

Exogenous growth hormone administration does not inhibit the growth hormone response to hexarelin in normal men.

Cappa. M M; Setzu. S S; Bernardini. S S; Carta. D D; Federici. G G; Grossi. A A; Loche. S S

In healthy men, giving a dose of growth hormone (GH) does not stop the GH‑boosting effect of the peptide hexarelin (a close relative of GHRP‑6). Even after a GH injection, hexarelin still caused a strong rise in blood GH levels, likely because it blocks somatostatin, the hormone that normally tells the pituitary to stop making GH.

Utility 4
pubmed 1996

Impaired growth hormone secretion in obese subjects is partially reversed by acipimox-mediated plasma free fatty acid depression.

Cordido. F F; Peino. R R; Peñalva. A A; Alvarez. C V CV; Casanueva. F F FF; Dieguez. C C

In obese people, high blood fats (free fatty acids) blunt the growth hormone (GH) boost you get from secretagogues like GHRP‑6. Lowering those fats with a drug called acipimox didn’t raise GH on its own, but when the drug was taken before GH‑stimulating agents, the GH spikes were much larger – even the strongest combo (GHRH + GHRP‑6) saw a ~50% increase. This suggests that cutting free fatty acids first can make GHRP‑6 work better.

Utility 4
pubmed 1996

Acipimox-mediated plasma free fatty acid depression per se stimulates growth hormone (GH) secretion in normal subjects and potentiates the response to other GH-releasing stimuli.

Peino. R R; Cordido. F F; Peñalva. A A; Alvarez. C V CV; Dieguez. C C; Casanueva. F F FF

Lowering blood free fatty acids (FFA) – for example by fasting, a low‑carb diet, or a drug like acipimox – makes the pituitary release more growth hormone on its own and dramatically boosts the GH spikes you get from GHRH, GHRP‑6, or a combo of both. The effect is additive and works no matter which GH‑releasing stimulus you use.

Utility 4
pubmed Nov 1, 1997

Hypothalamic targets for growth hormone secretagogues.

Robinson. I C IC

The study shows that growth‑hormone secretagogues like GHRP‑6 don’t just pull GH out of the pituitary – they also work in the brain, need a working GHRH system, and can boost cortisol. How you give the peptide (timing and pattern) changes the GH response, and the drugs can affect other hormones too.

Utility 4
pubmed 1999

A New Orphan Receptor Involved in Pulsatile Growth Hormone Release.

Smith. RG RG; Feighner. S S; Prendergast. K K; Guan. X X; Howard. A A

Scientists discovered that a special receptor called GHS‑R (the ghrelin or GH secretagogue receptor) is the key switch that makes the pituitary release growth hormone in bursts. This receptor is turned on by the GH‑releasing peptides like GHRP‑6, and activating it can boost the GH/IGF‑1 system, especially in older people where the natural signal fades.

Utility 4
pubmed Feb 1, 1996

Effect of intravenous or intracerebroventricular injections of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 on GH release in conscious, freely moving male rats.

Yagi. H H; Kaji. H H; Sato. M M; Okimura. Y Y; Chihara. K K

In rats, giving GHRP‑6 directly into the brain actually blocks growth hormone (GH) release, likely by boosting somatostatin (the hormone that stops GH). But when GHRP‑6 is injected into the bloodstream, it raises GH, especially when the natural somatostatin brake is removed or when a GHRH‑like drug is added, and the two together work better than either alone.

Utility 4
pubmed 1991

Desensitization studies using perifused rat pituitary cells show that growth hormone-releasing hormone and His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 stimulate growth hormone release through distinct receptor sites.

Blake. A D AD; Smith. R G RG

The study shows that the peptide GHRP‑6 makes the pituitary release growth hormone quickly, but the effect fades fast and the cells need about an hour to become fully responsive again. GHRP‑6 works through a different receptor than the usual growth‑hormone‑releasing hormone (GHRH), and using both together can give a bigger hormone surge that isn’t completely blocked by somatostatin.

Utility 4
pubmed 1994

Growth hormone (GH)-releasing peptide stimulation of GH release from human somatotroph adenoma cells: interaction with GH-releasing hormone, thyrotropin-releasing hormone, and octreotide.

Renner. U U; Brockmeier. S S; Strasburger. C J CJ; Lange. M M; Schopohl. J J; Müller. O A OA; v...

The study shows that the peptide GHRP‑6 reliably triggers growth hormone (GH) release from human pituitary tumor cells, even more consistently than the natural GH‑releasing hormone (GHRH) or thyroid‑releasing hormone (TRH). When GHRP‑6 is used together with GHRH or TRH, the GH boost adds up, while drugs that block somatostatin (like octreotide) can blunt its effect. The findings also point to a different receptor and a protein‑kinase‑C (PKC) pathway behind GHRP‑6’s action.

Utility 4
pubmed 1992

Acute growth hormone (GH) response to GH-releasing hexapeptide in humans is independent of endogenous GH-releasing hormone.

Robinson. B M BM; Friberg. R D RD; Bowers. C Y CY; Barkan. A L AL

The study shows that the synthetic peptide GHRP‑6 can trigger a rapid rise in growth hormone (GH) in humans even when the body's natural GH‑releasing hormone (GHRH) pathway is turned off. In a small test with five healthy men, giving GHRP‑6 after a 6‑hour GHRH infusion still produced a strong GH spike, proving that GHRP‑6 works through a different mechanism.

Utility 4
pubmed 1993

Massive growth hormone (GH) discharge in obese subjects after the combined administration of GH-releasing hormone and GHRP-6: evidence for a marked somatotroph secretory capability in obesity.

Cordido. F F; Peñalva. A A; Dieguez. C C; Casanueva. F F FF

In obese people, a synthetic peptide called GHRP‑6 makes the body release a lot of growth hormone, even more than the usual hormone‑releasing signal (GHRH). When GHRP‑6 is taken together with GHRH, the GH surge is huge, and a drug that boosts nerve signaling (pyridostigmine) can double the effect of GHRP‑6 alone. This shows that the low GH seen in obesity isn’t permanent and can be turned on again with the right mix of compounds.

Utility 4
pubmed 1991

Robust growth hormone (GH) secretion in aged female rats co-administered GH-releasing hexapeptide (GHRP-6) and GH-releasing hormone (GHRH).

Walker. R F RF; Yang. S W SW; Bercu. B B BB

In older female rats, giving the GH‑secretagogue GHRP‑6 alone didn’t boost growth hormone much, but when it was combined with a GH‑releasing hormone (GHRH) peptide, the GH surge was actually bigger than in young rats. This shows that the aging pituitary can still release a lot of GH if it gets the right mix of signals, meaning the age‑related drop in GH is more about weak stimulation than a broken gland.

Utility 4
pubmed 1996

Growth hormone response to GHRH, GHRP-6 and GHRH + GHRP-6 in patients with polycystic ovary syndrome.

Micić. D D; Kendereski. A A; Popović. V V; Sumarac. M M; Zorić. S S; Macut. D D; D...

The study shows that in women with PCOS who are obese, their growth hormone (GH) response to the usual GH‑releasing hormone (GHRH) is weak, but giving the peptide GHRP‑6 restores the GH surge, and combining GHRP‑6 with GHRH gives an even bigger boost. This means GHRP‑6 can overcome obesity‑related GH resistance and works well together with GHRH.

Utility 3
pubmed Aug 20, 2021

Molecular recognition of an acyl-peptide hormone and activation of ghrelin receptor.

Wang. Yue Y; Guo. Shimeng S; Zhuang. Youwen Y; Yun. Ying Y; Xu. Peiyu P; He. Xinheng X; Guo. Jia J;...

Scientists solved the 3‑D shape of the ghrelin receptor when it’s hooked up to the natural hunger hormone (ghrelin) and to a lab‑made peptide called GHRP‑6. They discovered a special pocket that grabs the tiny fatty‑acid tail on ghrelin, which is needed for the hormone to turn the receptor on. This explains how both ghrelin and GHRP‑6 activate the same pathway.

Utility 3
pubmed May 30, 2022

Antagonization of Ghrelin Suppresses Muscle Protein Deposition by Altering Gut Microbiota and Serum Amino Acid Composition in a Pig Model.

Yan. Xiaoxi X; Zhang. He H; Lin. Ailian A; Su. Yong Y

Blocking ghrelin with a special version of GHRP‑6 in pigs made them grow slower, reduced muscle size, lowered key amino acids in the blood, and changed gut bacteria that produce acetate. These changes also turned down the mTOR pathway (which builds muscle) and turned up genes that promote cell cleanup, leading to less muscle protein being deposited.

Utility 3
pubmed May 2, 2025

A novel butyrylcholinesterase inhibitor induces antidepressant, pro-cognitive, and anti-anhedonic effects in Flinders Sensitive Line rats: The role of the ghrelin-dopamine cascade.

Olivier. Nadia N; Harvey. Brian H BH; Gobec. Stanislav S; Shahid. Mohammed M; Košak. Urban U;...

In a rat model of depression, a new drug that blocks an enzyme called butyrylcholinesterase lifted mood, reward feeling, and memory performance. It did this by raising the levels of the hunger hormone ghrelin and dopamine in the brain. When researchers blocked the ghrelin receptor with a peptide (D‑Lys‑3‑GHRP‑6), all the good effects disappeared, showing that ghrelin signaling is key to the drug’s benefits.

Utility 3
pubmed May 30, 2024

Growth hormone releasing peptide-6 (GHRP-6) prevents doxorubicin-induced myocardial and extra-myocardial damages by activating prosurvival mechanisms.

Berlanga-Acosta. Jorge J; Cibrian. Danay D; Valiente-Mustelier. Juan J; Suárez-Alba. José...

In a rat study, giving the peptide GHRP-6 at the same time as the chemotherapy drug doxorubicin stopped the heart from getting damaged and also protected other organs. The peptide helped keep heart muscle cells healthy, reduced scar tissue, boosted the body's antioxidant defenses, and kept the cell's power plants (mitochondria) intact.

Utility 3
pubmed Feb 18, 2019

New Metabolic Influencer on Oxytocin Release: The Ghrelin.

Szabó. Renáta R; Ménesi. Rudolf R; H Molnár. Andor A; Szalai. Zita Z; Daruka. Le...

In rats, giving ghrelin (the hormone that GHRP‑6 mimics) either directly into the brain or into the bloodstream raises the amount of oxytocin in the blood. Blocking ghrelin with the antagonist [d‑Lys³]‑GHRP‑6 cuts this oxytocin boost. This shows that the ghrelin system can directly trigger oxytocin release.