Bioactive Polysaccharides from Fermented <i>Dendrobium officinale</i>: Structural Insights and Their Role in Skin Barrier Repair.
Wang. Wanshuai W; Zou. Anqi A; Yu. Qingtao Q; Wang. Zhe Z; Tan. Daotong D; Yang. Kaiye K; Cai. Chao C; Yu. Guangli G
Key Findings
- FDOP-1A and FDOP-2A significantly lowered inflammatory markers (MCP-1, TNF-α, NO, IL-1β) in LPS‑stimulated macrophage and keratinocyte cells.
- The extracts restored levels of key skin barrier proteins such as filaggrin, aquaporin‑3, TRPV4, LL‑37, and adiponectin.
- They suppressed the TLR4/IκB‑α/NF‑κB/NLRP3 signaling pathway and showed strong ROS‑scavenging (antioxidant) activity.
Practical Outcomes
- These findings suggest fermented Dendrobium polysaccharides could be explored as oral supplements or topical agents to improve skin barrier function and reduce inflammation, which may benefit anti‑aging or skin‑repair protocols. However, without human trials, optimal dosage, safety, and formulation remain uncertain, so biohackers should treat this as a promising lead rather than a ready‑to‑use solution.
Summary
Researchers found that two fermented Dendrobium polysaccharide extracts can calm inflammation and boost skin‑protective proteins in lab cells, while also acting as powerful antioxidants. This points to their potential as skin‑health ingredients, but human studies and dosing guidelines are still missing.
Abstract
<i>Dendrobium</i>, a prominent genus in the <i>Orchidaceae</i> family, has generated significant research attention due to its demonstrated biological potential, particularly its notable anti-inflammatory and antioxidant activities. In this study, two fractions of fermented <i>Dendrobium officinale</i> polysaccharides (FDOPs) were successfully isolated through a multi-stage purification strategy including gradient ethanol precipitation, gel column chromatography, and ion exchange chromatography with <i>Lactobacillus reuteri</i> CCFM863. Structural characterization revealed that both <i>Dendrobium officinale</i> polysaccharide fractions consisted of (1→4)-β-D-Man<i>p</i>, (1→4)-β-D-Glc<i>p</i>, and (1→4)-α-D-Glc<i>p</i> residues. The anti-inflammatory efficacy and keratinocyte-protective potential of FDOPs (FDOP-1A and FDOP-2A) were investigated by using lipopolysaccharide (LPS)-induced RAW264.7 and HaCaT cells models, which showed significant inhibitions on the inflammatory factors of monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α), nitric oxide (NO), and interleukin-1 beta (IL-1β); recovered levels of filaggrin (FLG), aquaporin 3 (AQP3), transient receptor potential vanilloid 4 (TRPV4), cathelicidin antimicrobial peptide (CAMP)/LL-37, and adiponectin (ADIPOQ); and the reduced protein expression of the TLR4/IκB-α/NF-κB/NLRP3 pathway. Notably, the FDOPs exhibited a remarkable reactive oxygen species (ROS) scavenging capacity, demonstrating superior antioxidant activity. Therefore, FDOPs show dual anti-inflammatory and antioxidant properties, making them suitable as active ingredients for modulating epidermal inflammation and promoting skin barrier repair.
Study Information
pubmed
2025
2025-07-06T00:00:00.000Z
10.3390/molecules30132875
2
49