Vitamin D3 (the form you get from sunlight or supplements) boosts the production of the antimicrobial peptide LL‑37 in mouth‑lining cells and reduces inflammation, helping these cells fight gum‑disease bacteria better. The study was done in a lab dish, so while it hints that good vitamin D levels could support oral health, real‑world effects still need human testing.

The oral epithelium is essential for maintaining oral health and plays a key role in the onset and progression of periodontitis. It serves as both a mechanical and immunological barrier and possesses antimicrobial activity. Vitamin D₃, a hormone with known immunomodulatory functions, may influence oral epithelial responses. This study investigated the effects of two vitamin D₃ metabolites on key immunological and antimicrobial functions of oral epithelial cells, both under basal conditions and during bacterial challenge. Ca9-22 oral epithelial cells were treated with 1,25(OH)₂D₃ or 25(OH)D₃ in the presence or absence of <i>Tannerella forsythia</i>, <i>Fusobacterium nucleatum</i>, or <i>Porphyromonas gingivalis</i>. Inflammatory responses were assessed by measuring gene and protein expression of IL-1&#x3b2; and IL-8. Antimicrobial activity was evaluated via expression of LL-37, hBD-2, and hBD-3, as well as direct bacterial killing assays. Expression of epithelial integrity markers E-cadherin and ICAM-1 was also analyzed. Vitamin D₃ metabolites reduced IL-8 expression and significantly increased LL-37 expression and production in Ca9-22 cells. Both forms enhanced antimicrobial activity against all tested pathogens and modulated epithelial integrity markers. Vitamin D₃ positively regulates antimicrobial and barrier functions in oral epithelial cells, suggesting a potential role in supporting oral health and preventing periodontitis progression.