TiO<sub>2</sub>nanotubes-MoS<sub>2</sub>/PDA-LL-37 exhibits efficient anti-bacterial activity and facilitates new bone formation under near-infrared laser irradiation.
Jin. Mingchao M; Zhu. Juli J; Meng. Zhipeng Z; Jiang. Xuesheng X; Chen. Zhuo Z; Xu. Juntao J; Gao. Hongliang H; Zhu. Junkun J; Wu. Fengfeng F
Key Findings
- A TiO2 nanotube surface coated with MoS2/PDA‑LL‑37 (T‑M/P‑L) improves mesenchymal stem cell adhesion, proliferation, alkaline phosphatase activity, collagen secretion, and mineralization.
- Near‑infrared (NIR) laser exposure activates the coating to provide strong antibacterial effects against common pathogens.
- The same NIR‑activated coating also promotes blood‑vessel‑forming signals (VEGF) and new bone formation in vivo.
Practical Outcomes
- For biohackers, the study shows that LL‑37 can work as part of a surface coating when combined with light activation, but there is no direct protocol you can use at home. It points to future implant technologies that might be safer and heal faster, rather than an actionable supplement or DIY treatment.
Summary
Scientists made a special coating for titanium implants that includes the antimicrobial peptide LL‑37, a molybdenum sulfide layer, and a sticky polymer. When they shine near‑infrared light on it, the coating kills bacteria and helps bone cells grow, even in animal tests. The work is mostly about improving medical implants, not something you can take or apply yourself.
Abstract
Titanium dioxide (TiO<sub>2</sub>), as one of the titanium (Ti)-based implants, holds a promise for a variety of anti-bacterial application in medical research. In the current study, a functional molybdenum disulfide (MoS<sub>2</sub>)/polydopamine (PDA)-LL-37 coating on titanium dioxide (TiO<sub>2</sub>) implant was prepared. Anodic oxidation and hydrothermal treatment was given to prepare TiO<sub>2</sub>nanotubes-MoS<sub>2</sub>/PDA-LL-37 (T-M/P-L). The<i>in vitro</i>osteogenic effect of T-M/P-L was evaluated by measuring mesenchymal stem cell (MSC) adhesion, proliferation, alkaline phosphatase (ALP) activity, extracellular matrix (ECM) mineralization, collagen secretion and osteoblast-specific messenger RNAs (mRNAs) expression. The determination on the anti-bacterial ability of T-M/P-L was followed. Furthermore, the ability of T-M/P-L to promote bone formation<i>in vivo</i>was evaluated. Near-infrared (NIR) laser irradiation exposure enabled the T-M/P-L coating-endowed Ti substrates to hold effective anti-bacterial ability. T-M/P-L promoted the adhesion and proliferation of MSCs. In addition, an increase was witnessed regarding the ALP activity, collagen secretion and ECM mineralization, along with the expression of runt-related transcription factor 2, ALP and osteocalcin in the presence of T-M/P-L. Additionally, T-M/P-L could stimulate endothelial cells to secrete vascular endothelial growth factor (VEGF) and promote capillary-like tubule formation. Upon NIR laser irradiation exposure, T-M/P-L not only exhibited efficient<i>in vivo</i>anti-bacterial activity but also facilitated new bone formation. Collectively, T-M/P-L had enhanced anti-bacterial and osteogenic activity under NIR laser irradiation.
Study Information
pubmed
2022
2022-06-24T00:00:00.000Z
10.1088/1748-605x/ac6470