Evaluation of the T-SPOT.TB test, oxidation-related factors, and antimicrobial peptide LL-37 in the diagnosis of pulmonary tuberculosis with type 2 diabetes.
Ma. Liangliang L; Chen. Xuelin X; Weng. Shengfeng S; Yang. Xinting X
Key Findings
- T‑SPOT.TB test (CFP10 and ESAT‑6 panels) shows high accuracy (AUC ~0.89) for TB in diabetics
- Combining CFP10 and ESAT‑6 gives the best performance (sensitivity 87.73%, specificity 88.93%, AUC 0.942)
- Patients with TB and diabetes have lower antioxidant enzymes and the addition of LL‑37 improves diagnostic value
Practical Outcomes
- For biohackers, the findings don’t translate into a new supplement or protocol. It mainly shows LL‑37 could be a useful diagnostic marker, but there’s no evidence it boosts immunity or longevity when taken as a supplement. So, no immediate actionable steps for self‑directed health optimization.
Summary
The study looked at how well a blood test (T-SPOT.TB) and some blood chemicals, including the immune peptide LL‑37, can detect lung TB in people who also have type‑2 diabetes. It found the test is pretty accurate, especially when two parts of it are combined, and that certain antioxidant levels are lower in these patients. However, the research is about diagnosing disease, not about using LL‑37 for health improvement or longevity.
Abstract
To investigate the diagnostic value of the T cell spot (T-SPOT.TB) test, oxidation-related factors (ORF), and antimicrobial peptide LL-37 in patients with pulmonary tuberculosis (PTB) with type 2 diabetes. A total of 560 patients with PTB admitted to our hospital from January 2019 to April 2021 were retrospectively included in this study. Of these, 232 patients with PTB and type 2 diabetes were assigned to the combined group, and 328 patients without complications were assigned to the PTB group. Areas under the curve (AUCs) for the number of spot-forming cells in CFP10 and ESAT-6 test panels detecting PTB with type 2 diabetes were 0.892 (95% confidence interval [CI] 0.831-0.921) and 0.893 (95% CI 0.841-0.935), respectively. CFP10 combined with ESAT-6 had the highest diagnostic value, with sensitivity and specificity levels and an AUC of 87.73%, 88.93%, and 0.942 (95% CI 0.907-0.967), respectively. The levels of total antioxidant capacity, superoxide dismutase, and catalase in the combined group were lower than in PTB and control groups. The combination of T-SPOT.TB, ORF, and LL-37 in the diagnosis of pulmonary tuberculosis with type 2 diabetes mellitus has a high diagnostic value and clinical application value.
Study Information
pubmed
2021
2021-12-01T00:00:00.000Z
10.1177/03000605211064418
1
32