The study shows that skin‑friendly bacteria (healthy P. acnes strains) trigger immune cells (Th17) that release LL‑37, but LL‑37 alone doesn’t kill the acne‑causing bacteria. Instead, other secreted factors from these immune cells are responsible for the antibacterial effect.

Studies of the human skin microbiome suggest that Propionibacterium acnes strains may contribute differently to skin health and disease. However, the immune phenotype and functions of T helper type 17 (Th17) cells induced by healthy (P_H) versus acne (P_A) skin-associated P. acnes strains are currently unknown. We stimulated peripheral blood mononuclear cells from healthy donors and observed that P_A strains induce higher IL-17 levels than P_H strains. We next generated P_H and P_A strain-specific Th17 clones and show that P. acnes strains induce Th17 cells of varied phenotype and function that are stable in the presence of IL-2 and IL-23. Although P_H- and P_A-specific clones expressed similar levels of LL-37 and DEFB4, only P_H-specific clones secreted molecules sufficient to kill P. acnes. Furthermore, electron microscopic studies showed that supernatants derived from activated P_H and not P_A-specific clones exhibited robust bactericidal activity against P. acnes, and complete breaches in the bacterial cell envelope were observed. This antimicrobial activity was independent of IL-26, because both natural IL-26 released by Th17 clones and rhIL-26 lacked antimicrobial potency against P. acnes. Overall, our data suggest that P. acnes strains may differentially modulate the CD4⁺ T-cell responses, leading to the generation of Th17 cells that may contribute to either homeostasis or acne pathogenesis.