A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions.
Boesch. Austin W AW; Zhao. Yifeng Y; Landman. Alison S AS; Garcia. Marta Rodriguez MR; Fahey. John V JV; Wira. Charles R CR; Ackerman. Margaret E ME
Key Findings
- A multiplexed ELISA‑based assay can simultaneously quantify ten antimicrobial peptides, including LL‑37.
- The assay shows high reproducibility and linear detection across two orders of magnitude for most targets.
- It works with complex biological fluids such as cervicovaginal lavage and reduces cost and sample volume versus traditional single‑plex ELISAs.
Practical Outcomes
- For biohackers, this means you could now track your own LL‑37 and other immune peptides more easily using a single, affordable test. While it doesn’t tell you how to dose or use LL‑37, it provides a tool for monitoring changes in your mucosal immunity as part of a broader health‑optimization protocol.
Summary
Scientists created a test that can measure several immune‑boosting peptides, including LL‑37, at the same time in a single sample. The test is reliable, works over a wide range of concentrations, and can be used with real body fluids, saving time and money compared to doing many separate tests.
Abstract
Mucosal tissues represent the front line in defense against potential pathogens, and one means by which mucosa provide protection is via the secretion of antimicrobials which can interfere with potential pathogens as well as recruit and modify the responses of immune cells. Here we describe adaptation of ELISA assays to microsphere format, facilitating simultaneous quantification of antimicrobial peptides including elafin, MIP3α, HBD2, HBD3, SLPI, RANTES, SDF1, lactoferrin, LL-37, and HNP1-3. The multiplexed assay exhibits excellent reproducibility, shows linearity over a two order of magnitude concentration range for most analytes, is compatible with biological fluids such as cervicovaginal lavage fluid, and presents significant cost and sample savings relative to traditional ELISA assays.
Study Information
pubmed
2013
2013-09-11T00:00:00.000Z
10.1016/j.jim.2013.09.001
13
10