Localization of antimicrobial peptides on polymerized liposomes leading to their enhanced efficacy against Pseudomonas aeruginosa.
Kumar. Amit A; Kolar. Satya S SS; Zao. Meriong M; McDermott. Alison M AM; Cai. Chengzhi C
Key Findings
- IG‑25 (a truncated LL‑37) was linked to polymerized liposomes using click chemistry
- The liposome‑bound peptide was 18‑fold more effective against Pseudomonas aeruginosa than free LL‑37
- No additional toxicity was observed in human corneal epithelial cells
Practical Outcomes
- Using liposome carriers can dramatically boost the antimicrobial power of LL‑37 without extra safety risks. However, the required chemistry (click‑reaction, polymerized liposomes) is complex, so most DIY users would need a ready‑made product or collaboration with a lab to apply this insight.
Summary
The study shows that a shortened version of the LL‑37 peptide, when chemically attached to specially made liposome particles, kills the bacteria Pseudomonas aeruginosa about 18 times better than the free peptide, and it doesn’t hurt human eye cells any more.
Abstract
Antimicrobial peptide IG-25 (a truncated version of LL-37 of the cathelicidin family) tethering an azido-capped poly(ethylene glycol) chain at the N-terminus was site-specifically attached to alkynyl-terminated polymerized liposomes using copper catalyzed "click" reaction, leading to an 18 fold enhancement in efficacy against Pseudomonas aeruginosa when compared to LL-37 without any increase in cytotoxicity to human corneal epithelial cells.
Study Information
pubmed
2011
2011-01-13T00:00:00.000Z
10.1039/c0mb00207k
17
50