Sensitivity of Chlamydia suis to cathelicidin peptides.
Donati. Manuela M; Di Francesco. Antonietta A; Gennaro. Renato R; Benincasa. Monica M; Magnino. Simone S; Pignanelli. Salvatore S; Shurdhi. Alisa A; Moroni. Alessandra A; Mazzoni. Claudio C; Merialdi. Giuseppe G; Baldelli. Raffaella R; Cevenini. Roberto R
Key Findings
- SMAP‑29 reduced Chlamydia inclusion numbers at 10 µg/ml in most isolates
- Some isolates were resistant even at 80 µg/ml
- LL‑37 and PG‑1 showed no anti‑chlamydial effect at 80 µg/ml
Practical Outcomes
- LL‑37 isn’t effective against this Chlamydia strain, so it isn’t a viable antimicrobial supplement for this purpose. The results suggest focusing on other peptides if targeting similar bacteria, but the findings have limited direct relevance to human health or typical biohacking protocols.
Summary
The study tested several natural antimicrobial peptides, including LL‑37, against a pig‑related Chlamydia infection. LL‑37 didn’t work even at high doses, while another peptide (SMAP‑29) showed some activity. For most biohackers, this doesn’t give a useful new health hack.
Abstract
Nine Chlamydia suis isolates, obtained from pigs with conjunctivitis, were molecularly characterized by ompA sequencing and their in vitro susceptibility to six cathelicidin peptides (SMAP-29, BAC-7, BMAP-27, BMAP-27, BMAP-28, PG-1, LL-37) determined in cell culture. SMAP-29 was the most active peptide, reducing the intracellular inclusion number by > or =50% at a concentration of 10 microg/ml (3 microM) in six of the nine isolates tested. Three molecularly identical isolates were insensitive at a concentration as high as 80 microg/ml (25 microM). Of the remaining cathelicidin peptides tested, BAC-7 and BMAP-27 were active against six C. suis isolates at a concentration of 80 microg/ml (25 and 26 microM, respectively). Cathelicidins LL-37 and PG-1 did not show any anti-chlamydial activity at 80 microg/ml.
Study Information
pubmed
2007
2007-02-16T00:00:00.000Z
10.1016/j.vetmic.2007.02.011