Effect of bioregulatory tripeptides on the culture of skin cells from young and old rats.
Voicekhovskaya. M A MA; Chalisova. N I NI; Kontsevaya. E A EA; Ryzhak. G A GA
Key Findings
- Pinealon (Glu‑Asp‑Ala) increased skin cell proliferation in young rat skin explants.
- In old rat skin, only the Lys‑Glu‑Asp peptide showed a strong proliferative effect; pinealon did not.
- Higher cell numbers were linked to reduced expression of the pro‑apoptotic protein p53, indicating less cell death.
Practical Outcomes
- For DIY biohackers, the data suggest pinealon might aid skin regeneration, but only in younger tissue and only in animal models. There’s no dosage, delivery method, or human safety information, so it’s not ready for a reliable protocol. If you’re interested in skin health, focus on well‑studied ingredients and treat this as very early, exploratory evidence.
Summary
In rats, a synthetic version of the pinealon tripeptide (Glu‑Asp‑Ala) helped skin cells grow faster in young animals, but it didn’t have the same effect in older rats. Another peptide (Lys‑Glu‑Asp) worked better in old skin. The boost in cell growth seemed to come from less cell death, not just more cell division.
Abstract
We studied the effects of synthesized tripeptides T-32 (Glu-Asp-Ala), T-33 (Glu-Asp-Arg), T-34 (Glu-Asp-Gly), T-36 (Glu-Asp-Pro), and T-38 (Lys-Glu-Asp) on organotypic skin cultures of young and old rats. In skin explants from young rats, all peptides except T-34 produced a stimulating effect on cell proliferation. In skin explants from old rats, tripeptide T-38 produced a marked stimulatory effect on proliferation. Immunocytochemical study of the proapoptotic p53 protein expression showed that cell proliferation increased due to less pronounced apoptosis. The capacity of the studied tripeptides to promote cell proliferation in the skin tissues of young and old animals provides the basis for further study of these substances as preparations boosting the regenerative processes in the skin, including those at age-associated pathology.
Study Information
pubmed
2012
2012-01-01T00:00:00.000Z
10.1007/s10517-012-1527-9