[POLYPEPTIDES INFLUENCE ON TISSUE CELL CULTURES REGENERATION OF VARIOUS AGE RATS].
Ryzhak. A P AP; Chalisova. N I NI; Lin'kova. N S NS; Khalimov. R I RI; Ryzhak. G A GA; Zhekalov. A N AN
Key Findings
- Thymalin and similar peptides boost growth of young and old rat tissue cultures at 20‑50 ng/ml
- They raise PCNA (a proliferation marker) and lower p53 (a stress/tumor‑suppressor protein)
- Thymalin uniquely increases CD5 and CD20, indicating promotion of B‑cell differentiation
Practical Outcomes
- The data hint that Thymalin could aid tissue regeneration and support immune cell formation, but the work is limited to rat cells in a dish. No human dosing or safety info is available, so it’s not ready for direct supplementation protocols. Enthusiasts should view this as early‑stage evidence requiring more research before practical use.
Summary
Researchers tested tiny amounts of Thymalin and other tissue‑derived peptides on rat brain, liver, heart and other cell cultures from young and old animals. At 20‑50 ng/ml the peptides helped the cells grow, boosted a cell‑division marker (PCNA) and lowered a stress‑related protein (p53). Thymalin also increased markers of B‑cell development (CD5, CD20).
Abstract
A comparative study of polypeptides extracted from the tissues of calves: Cortexin (from brain cortex), Epinorm (from pineal gland), Ventvil (from liver), Prostatilen (from prostate), Thymalin (from thymus), Chelohart (from heart), Chondrolux (from cartilage) on the relevant organotypic tissue cultures of young and old rats, in concentration 0,01-100 ng/ml was performed. Polypeptides specifically stimulated "young" and "old" cell cultures growth in concentration 20-50 ng/ml. This effect correlates with increasing of PCNA and decreasing of p53 expression in brain cortex, pineal gland, liver, prostate, heart, cartilage. Moreover, Thymalin activated CD5, CD20 expression--markers of B-cells differentiation. These data show that polypeptides isolated from different tissues have selective molecular activity on the regeneration of suitable tissues in aging.
Study Information
pubmed
2015