Protective effect of tripeptide in the presence of cyclophosphamide on the growth of cultured lymphoid tissue from rats of different age.
Chalisova. N I NI; Lesnyak. V V VV; Oganezova. E V EV; Rizhak. G A GA
Key Findings
- T-38 at 0.05 ng/ml boosted growth of rat spleen tissue explants
- Cyclophosphamide (1 mg/ml) halted cell proliferation and raised p53 levels
- When T-38 and cyclophosphamide were combined, the drug’s suppressive effect disappeared, indicating a protective effect
Practical Outcomes
- The result is interesting but not ready for any DIY health protocol. It shows a possible way to protect immune cells from chemotherapy in rats, but there’s no human data, dosing guidance, or safety info. Biohackers should wait for more research before considering any use.
Summary
A tiny protein made of three amino acids (Lys-Glu-Asp) helped rat spleen cells keep growing even when a chemotherapy drug that normally stops cell growth was added, but this was only shown in a lab dish with rat tissue, not in people.
Abstract
We studied the effect of tripeptide T-38 (Lys-Glu-Asp) in the presence of cyclophosphamide on cell proliferation and apoptosis in explants of splenic lymphoid tissue from young and old rats. Peptide T-38 in a concentration of 0.05 ng/ml produced a stimulatory effect on the growth zone of the explants. Addition of 1 mg/ml cyclophosphamide to the culture medium suppressed cell proliferation, which was associated with enhanced expression of proapoptotic p53 protein. Under conditions of combined treatment with cyclophosphamide and T-38 no inhibiting effect of the cytostatics was observed. Thus, tripeptide T-38 in the presence of cytostatics produces a protective effect on cell proliferation in lymphoid tissue explants.
Study Information
pubmed
2008
2008-10-25T00:00:00.000Z
10.1007/s10517-008-0189-0
3
9