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Thymosin-beta-4-fragment

Ac-SDKP, Goralatide, Seraspenide

A tetrapeptide fragment of thymosin beta-4 that inhibits inflammation, reduces fibrosis, and regulates hematopoietic stem cell proliferation.

Quick Stats
Studies 83
Trials 3
Formula C20H33N5O9
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Utility 1
pubmed 2003

Reduction of thymosin beta4 and actin in HL60 cells during apoptosis is preceded by a decrease of their mRNAs.

Müller. Christian S G CS; Huff. Thomas T; Hannappel. Ewald E

In a lab study, a drug called araC caused human leukemia cells to die, and as they did, the levels of a small protein called thymosin‑beta‑4 and the building block actin both fell dramatically, along with their mRNA messages. This shows that during cell death, the cells stop making these proteins, but the experiment was done in cell culture, not people.

Utility 1
pubmed May 6, 1996

The N-terminal sequences (5-20) of thymosin beta 4 binds to monomeric actin in an alpha-helical conformation.

Feinberg. J J; Heitz. F F; Benyamin. Y Y; Roustan. C C

The study shows that a short piece of the protein thymosin‑beta‑4 (amino acids 5‑20) is floppy in water but folds into a helix when mixed with a lab chemical called TFE, and this folded shape sticks to single actin molecules much better. The chemical doesn’t change actin itself.

Utility 1
pubmed Mar 26, 1999

Dimerization of profilin II upon binding the (GP5)3 peptide from VASP overcomes the inhibition of actin nucleation by profilin II and thymosin beta4.

Jonckheere. V V; Lambrechts. A A; Vandekerckhove. J J; Ampe. C C

The study shows that a small peptide from the protein VASP can stick to another protein called profilin II, forming a dimer that cancels out the normal slowdown of actin building caused by profilin II and thymosin‑beta‑4, but this effect only happens in a test‑tube setting and doesn’t translate into a clear health or performance protocol.

Utility 1
pubmed Aug 28, 2025

Electron Capture Dissociation for Discovery Top-Down Proteomics of Peptides and Small Proteins on Chromatographic Time Scales.

Manly. Lester S LS; Roberts. Anne M AM; Beckman. Joseph S JS; Roberts. Blaine R BR

The paper describes a fancy mass‑spectrometry technique that can read the exact sequence and tiny chemical changes of small proteins, including thymosin‑beta‑4, but it doesn’t give any advice on how to use the peptide for health or performance.

Utility 1
pubmed 2005

Top-down identification of endogenous peptides up to 9 kDa in cerebrospinal fluid and brain tissue by nanoelectrospray quadrupole time-of-flight tandem mass spectrometry.

Möhring. Thomas T; Kellmann. Markus M; Jürgens. Michael M; Schrader. Michael M

Scientists showed a new, efficient way to spot tiny proteins like thymosin‑beta‑4 directly in brain tissue and spinal fluid using advanced mass‑spectrometry, but the study doesn’t give any dosage or usage tips for people.

Utility 1
pubmed Sep 18, 2008

Increased plasma concentrations of cytoskeletal and Ca2+-binding proteins and their peptides in psoriasis patients.

Plavina. Tatiana T; Hincapie. Marina M; Wakshull. Eric E; Subramanyam. Meena M; Hancock. William S W...

The study found that people with psoriasis have higher levels of certain cell‑structure proteins and their tiny fragments, including pieces of the peptide thymosin‑beta‑4, in their blood. This likely reflects skin cell damage and increased protein breakdown, not a health‑boosting effect you can copy with supplements.

Utility 1
pubmed Dec 1, 1994

Interaction of plant profilin with mammalian actin.

Giehl. K K; Valenta. R R; Rothkegel. M M; Ronsiek. M M; Mannherz. H G HG; Jockusch. B M BM

Scientists found that a plant protein called birch profilin can stick to animal actin proteins in a way similar to animal profilin, and it competes with the peptide thymosin‑beta‑4 for the same spot on actin. The binding is weak (micromolar) and depends on magnesium, but the study is mostly basic science and doesn’t give any direct health or performance tips.

Utility 1
pubmed Aug 11, 2015

Distinct actin oligomers modulate differently the activity of actin nucleators.

Qu. Zheng Z; Silvan. Unai U; Jockusch. Brigitte M BM; Aebi. Ueli U; Schoenenberger. Cora-Ann CA; Man...

Scientists made tiny clusters of actin proteins and found that each type changes how actin filaments grow, and they interact differently with proteins like gelsolin, formins, Arp2/3, and the peptide thymosin‑β4. The findings are mostly basic cell‑biology and don’t give clear steps you can use in a health‑oriented routine.

Utility 1
pubmed Apr 6, 2001

Actin filament cross-linking by MARCKS: characterization of two actin-binding sites within the phosphorylation site domain.

Yarmola. E G EG; Edison. A S AS; Lenox. R H RH; Bubb. M R MR

The study shows that a protein called MARCKS has two spots that bind to actin, and these spots compete with the natural peptide thymosin‑beta‑4 for binding, but the work is purely mechanistic and doesn’t give any direct advice on using thymosin‑beta‑4 as a supplement.

Utility 1
pubmed May 29, 2015

Increases in plasma Tβ4 after intracardiac cell therapy in chronic ischemic heart failure is associated with symptomatic improvement.

Choudry. Fizzah A FA; Yeo. Chia C; Mozid. Abdul A; Martin. John F JF; Mathur. Anthony A

In a tiny study of people with chronic heart failure, injecting bone‑marrow stem cells into the heart caused a short‑term rise in a natural protein called thymosin‑beta‑4, and those who showed the biggest rise also felt less short‑of‑breath. The work is early and uses a surgical procedure, so it doesn’t give a clear way for everyday health‑hackers to use the peptide.

Utility 1
pubmed Nov 8, 2011

Controlling charge states of peptides through inductive electrospray ionization mass spectrometry.

Peng. Yue'e Y; Zhang. Sichun S; Gong. Xiaoyun X; Ma. Xiaoxiao X; Yang. Chengdui C; Zhang. Xinrong X

Scientists made a new tweak to the electrospray ionization tool that lets them change how many electric charges a peptide carries when it’s measured, simply by adjusting the voltage. This makes the mass‑spectrometry readout simpler, showing mostly single‑charged peptide ions and fewer confusing extra signals. While it’s a neat technical improvement for labs, it doesn’t change how you would take or use thymosin‑beta‑4‑fragment in everyday biohacking.

Utility 1
pubmed Aug 5, 1991

Rat thymosin beta 4 gene. Intron-containing gene and multiple retroposons.

Varghese. S S; Kronenberg. H M HM

Researchers discovered that rats have many thymosin‑beta‑4‑like sequences that are retroposons (no introns) and one regular gene with two introns and a promoter, showing how the gene is organized but giving no clues for using the peptide in humans.

Utility 1
pubmed Jan 25, 1982

Chemical characterization of thymosin beta 4.

Low. T L TL; Goldstein. A L AL

Scientists figured out the exact makeup of a tiny protein called thymosin beta‑4, including its size, charge, and that it has an acetyl group at the start. They showed it can boost a specific enzyme in mouse immune cells and can slow down macrophage movement, but they didn’t test it in people or give any usage guidelines.

Utility 1
pubmed Sep 1, 1990

Expression of the thymosin beta 4 gene during differentiation of hematopoietic cells.

Shimamura. R R; Kudo. J J; Kondo. H H; Dohmen. K K; Gondo. H H; Okamura. S S; Ishibashi. H H; Niho....

This study looked at how the gene for thymosin‑beta‑4 behaves in blood‑cancer cells and found it turns on more when those cells start to change into more mature types, especially in certain T‑cell cancers.

Utility 1
pubmed Jun 15, 1992

Phosphoinositide-binding peptides derived from the sequences of gelsolin and villin.

Janmey. P A PA; Lamb. J J; Allen. P G PG; Matsudaira. P T PT

The study looked at short lab‑made pieces of two proteins (gelsolin and villin) that can stick to certain cell‑membrane fats called phosphoinositides. These pieces can pull the fats away from the full proteins, letting the proteins cut actin filaments again. The natural peptide thymosin‑beta‑4 was tested but showed much weaker effects, meaning it isn’t a good stand‑in for these specially designed peptides.

Utility 1
pubmed Sep 1, 1983

Thymosin beta 10, a new analog of thymosin beta 4 in mammalian tissues.

Erickson-Viitanen. S S; Ruggieri. S S; Natalini. P P; Horecker. B L BL

Scientists found a new peptide called thymosin beta 10 that is similar to the well‑known thymosin beta 4. It is present in many animal tissues at very low levels, but the study only describes its structure and where it’s found, not what it does or how to use it.

Utility 1
pubmed May 1, 2010

Thymosin beta4 and its posttranslational modifications.

Hannappel. E E

The paper explains how the protein thymosin beta‑4 is chemically modified inside the body (acetylation, phosphorylation, cross‑linking, and cutting into smaller pieces). It points out that we still don’t know how much of these modified forms exist in cells or what they actually do, and that a fragment of the protein is higher in people with rheumatoid arthritis.

Utility 1
pubmed 1997

A novel beta-thymosin from the sea urchin: extending the phylogenetic distribution of beta-thymosins from mammals to echinoderms.

Stoeva. S S; Hörger. S S; Voelter. W W

Scientists found a new version of the peptide thymosin‑beta, called thymosin beta‑14, in sea urchins. It’s a small protein of 40 amino acids that looks a lot like the well‑known thymosin beta‑4 from mammals, but the study only describes its structure, not any health effects.

Utility 1
pubmed Jun 23, 2016

A peptide from human β thymosin as a platform for the development of new anti-biofilm agents for Staphylococcus spp. and Pseudomonas aeruginosa.

Schillaci. Domenico D; Spinello. Angelo A; Cusimano. Maria Grazia MG; Cascioferro. Stella S; Barone....

Scientists tested a short piece of the human protein thymosin‑beta‑4 and found it can kill some bacteria and stop them from forming protective biofilms in lab dishes, but only at relatively high concentrations and only in test‑tube experiments.