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Humanin

HN, S14G-Humanin

A mitochondrial-derived peptide that protects against apoptosis, oxidative stress, and has potential in neurodegenerative and metabolic diseases.

Quick Stats
Studies 491
Trials 100
Formula C119H204N34O32S2
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Utility 2
pubmed Jul 20, 2017

Humanin G (HNG) protects age-related macular degeneration (AMD) transmitochondrial ARPE-19 cybrids from mitochondrial and cellular damage.

Nashine. Sonali S; Cohen. Pinchas P; Chwa. Marilyn M; Lu. Stephanie S; Nesburn. Anthony B AB; Kupper...

A lab study using eye‑cell models showed that a stronger form of the natural peptide Humanin (called HNG) can protect cells with mitochondria from people with age‑related macular degeneration (AMD). The peptide helped keep the cells alive, reduced stress signals, and lowered damage caused by a protein linked to AMD. This is early‑stage, cell‑culture work, not a human trial, but it points to Humanin‑based approaches as a possible future way to slow or prevent dry AMD.

Utility 2
pubmed Jan 18, 2020

Humanin gene expression in fibroblast of Down syndrome subjects.

Salemi. Michele M; Ridolfo. Federico F; Salluzzo. Maria Grazia MG; Cannarrella. Rossella R; Giambirt...

Researchers found that cells taken from people with Down syndrome make more of a tiny protein called humanin, which is known to protect cells from stress. This suggests humanin might be part of why Down syndrome cells behave differently, but the study doesn’t test any treatments or give dosing advice.

Utility 2
pubmed Mar 16, 2020

Humanin analogue, HNG, inhibits platelet activation and thrombus formation by stabilizing platelet microtubules.

Ren. Lijie L; Li. Qing Q; You. Tao T; Zhao. Xuefei X; Xu. Xingshun X; Tang. Chaojun C; Zhu. Li L

A modified version of the anti‑Alzheimer peptide humanin, called HNG, was shown in mice to calm down platelets – the cells that help blood clot – and to prevent the formation of dangerous clots without making bleeding worse. It does this by keeping the tiny tubes inside platelets stable through a chemical change called tubulin acetylation.

Utility 2
pubmed Feb 10, 2018

Humanin promotes mitochondrial biogenesis in pancreatic MIN6 β-cells.

Qin. Qingqing Q; Jin. Jieqiong J; He. Fang F; Zheng. Yongqin Y; Li. Tingting T; Zhang. Yun Y; He. Ju...

The study found that the naturally occurring peptide humanin can boost the creation and function of mitochondria—the cell's power plants—in mouse pancreatic beta cells, mainly by turning on a key energy‑regulating pathway (AMPK). This leads to more mitochondrial DNA, higher ATP production, and better cell performance, but the work was done only in lab‑grown cells, not in people.

Utility 2
pubmed May 22, 2020

Humanin protects cortical neurons from calyculin A-induced neurotoxicities by increasing PP2A activity and SOD.

Zhao. Jinfeng J; Zeng. Yu Y; Wang. Yaxin Y; Shi. Junzhen J; Zhao. Wenhui W; Wu. Baoai B; Du. Huizhi...

The study shows that the peptide humanin can protect brain cells in a dish from a toxin that mimics damage seen in neurodegenerative diseases. It does this by keeping an enzyme called PP2A active, lowering oxidative stress, and preventing harmful changes to the tau protein.

Utility 2
pubmed Mar 28, 2018

Humanin (HN) and glucose transporter 8 (GLUT8) in pregnancies complicated by intrauterine growth restriction.

Janzen. Carla C; Lei. Margarida Y Y MYY; Jeong. Il Seok D ISD; Ganguly. Amit A; Sullivan. Peggy P; P...

The study found that the mitochondrial peptide humanin is higher in the part of the placenta that contacts the mother in pregnancies where the baby is not growing enough, and it rises when cells are low on oxygen. Humanin also sticks together with a sugar‑transport protein called GLUT8, and both go up under low‑oxygen conditions, but only humanin goes up when nutrients are scarce. These results show humanin reacts to stress in pregnancy tissue, but they don’t give any direct tips for using humanin in health‑hacking.

Utility 2
pubmed Feb 28, 2019

Humanin Nanoparticles for Reducing Pathological Factors Characteristic of Age-Related Macular Degeneration.

Solanki. Aum A; Smalling. Rudy R; Parola. Abraham H AH; Nathan. Ilana I; Kasher. Roni R; Pathak. Yas...

Scientists made a stronger version of the brain peptide humanin (called AGA‑HNG) and packed it into tiny chitosan particles. In lab tests on eye‑cell cultures, these particles lowered the harmful VEGF protein and protected cells from oxidative death, while being less toxic and releasing the drug more steadily than the plain peptide. The work shows the concept works, but it’s still early‑stage and only tested in dishes, not in people.

Utility 2
pubmed Jul 18, 2018

Humanin prevents high glucose-induced monocyte adhesion to endothelial cells by targeting KLF2.

Wang. Xiaohui X; Wu. Ziheng Z; He. Yangyan Y; Zhang. Hongkun H; Tian. Lu L; Zheng. Chengfei C; Shang...

Humanin, a tiny protein made in mitochondria, was shown in lab cells to protect blood‑vessel lining from high‑sugar damage. It does this by turning on a protective gene called KLF2, which then lowers inflammation signals and stops immune cells from sticking to the vessel walls.

Utility 2
pubmed 2018

Humanin Exerts Neuroprotection During Cardiac Ischemia-Reperfusion Injury.

Kumfu. Sirinart S; Charununtakorn. Savitree T ST; Jaiwongkam. Thidarat T; Chattipakorn. Nipon N; Cha...

In a rat study, giving a humanin‑like peptide (HNG) during a heart attack helped protect the brain by improving mitochondrial function and lowering markers linked to Alzheimer’s disease, like tau and amyloid‑beta, but it didn’t fix blood‑brain‑barrier leaks or oxidative stress. The effect was seen at medium‑high doses (168‑252 µg per kg) given intravenously at the time of the heart attack or right when blood flow returned.

Utility 2
pubmed Nov 16, 2018

Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family.

Nan. Aruo A; Chen. Lijian L; Zhang. Nan N; Jia. Yangyang Y; Li. Xin X; Zhou. Hanyu H; Ling. Yihui Y;...

Researchers found that a circular RNA called circNOL10 can increase levels of the humanin peptide family, which then slows the growth of lung cancer cells in lab experiments. The effect works through a chain of proteins that ultimately boost humanin and trigger cancer cell death, but the study was done in cells and mice, not people.

Utility 2
pubmed Aug 16, 2017

Humanin analogue, S14G-humanin, has neuroprotective effects against oxygen glucose deprivation/reoxygenation by reactivating Jak2/Stat3 signaling through the PI3K/AKT pathway.

Gao. Guang-Sheng GS; Li. Yun Y; Zhai. Heng H; Bi. Jing-Wen JW; Zhang. Fu-Sen FS; Zhang. Xiao-Ying XY...

A lab study found that a modified version of the tiny protein humanin, called S14G‑humanin (HNG), can protect brain‑like cells from damage that mimics a stroke, mainly by turning on a cell‑survival pathway (Jak2/Stat3 via PI3K/AKT). The effect was strongest at a very low concentration (1 µg/L) in a dish, but the work was done only in cells, not people.

Utility 2
pubmed Apr 4, 2017

Humanin inhibits apoptosis in pituitary tumor cells through several signaling pathways including NF-κB activation.

Gottardo. María Florencia MF; Moreno Ayala. Mariela M; Ferraris. Jimena J; Zárate. Sandra...

Humanin is a small protein that can protect pituitary cells from dying by turning on several cell‑survival pathways, but the same protection might also help tumor cells stay alive, so its safety for anti‑aging use isn’t clear yet.

Utility 2
pubmed Jul 26, 2017

Protective Mechanisms of the Mitochondrial-Derived Peptide Humanin in Oxidative and Endoplasmic Reticulum Stress in RPE Cells.

Minasyan. Leonid L; Sreekumar. Parameswaran G PG; Hinton. David R DR; Kannan. Ram R

The study shows that the tiny protein humanin can shield eye cells (RPE cells) from damage caused by oxidative stress and stress in the endoplasmic reticulum, which are linked to age‑related macular degeneration. It works by boosting the cell’s antioxidant glutathione, cutting down harmful ROS, and stopping cell‑death enzymes. While promising for eye health, the work is done in lab dishes, not people, so it isn’t a ready‑to‑use protocol yet.

Utility 2
pubmed Jul 17, 2025

The unexplored Nexus: Mitochondria derived microproteins and Parkinson's disease.

Patel. Kashish K; Soni. Ritu R; Shah. Jigna J

The paper reviews how tiny proteins made by mitochondria, especially Humanin and related peptides, might protect brain cells in Parkinson's disease by reducing inflammation, oxidative stress, and metabolic problems. It highlights that these peptides are a new research focus but does not give concrete dosing or treatment plans.

Utility 2
pubmed Sep 9, 2019

Mitochondrial Peptide Humanin Protects Silver Nanoparticles-Induced Neurotoxicity in Human Neuroblastoma Cancer Cells (SH-SY5Y).

Gurunathan. Sangiliyandi S; Jeyaraj. Muniyandi M; Kang. Min-Hee MH; Kim. Jin-Hoi JH

The study shows that the tiny protein humanin can shield brain‑derived cells from damage caused by silver nanoparticles, which are known to create oxidative stress and kill cells. In lab dishes, giving cells humanin before or together with the nanoparticles helped keep the cells alive, maintained their energy factories, and reduced DNA damage and cell death.

Utility 2
pubmed Jan 1, 2017

Endoplasmic reticulum-mitochondrial crosstalk: a novel role for the mitochondrial peptide humanin.

Sreekumar. Parameswaran G PG; Hinton. David R DR; Kannan. Ram R

The review says a tiny protein called humanin, which comes from mitochondria, can help protect cells from damage caused by oxidative stress and stress in the endoplasmic reticulum, especially in eye cells linked to age‑related macular degeneration. It works partly by restoring a key antioxidant (glutathione) in mitochondria. While promising, the findings are still at the cell‑culture stage and not yet ready for everyday use.

Utility 2
pubmed Aug 21, 2019

Reduced skeletal muscle expression of mitochondrial-derived peptides humanin and MOTS-C and Nrf2 in chronic kidney disease.

Liu. Chang C; Gidlund. Eva-Karin EK; Witasp. Anna A; Qureshi. Abdul Rashid AR; Söderberg. Magnu...

In people with end‑stage kidney disease, the muscle cells make less of the protective peptides humanin and MOTS‑C, while the blood shows more humanin but less MOTS‑C. Lower muscle levels are linked to fewer mitochondria and more oxidative stress, and higher blood humanin is tied to inflammation. This shows that kidney disease disrupts the balance of these mitochondria‑derived peptides, which are thought to help cells survive and manage glucose.

Utility 2
pubmed Oct 1, 2017

High-dose Humanin analogue applied during ischemia exerts cardioprotection against ischemia/reperfusion injury by reducing mitochondrial dysfunction.

Thummasorn. Savitree S; Shinlapawittayatorn. Krekwit K; Chattipakorn. Siriporn C SC; Chattipakorn. N...

In rats, a high dose of a Humanin‑like peptide given while the heart was blocked reduced heart rhythm problems, heart tissue damage, and helped the heart's power plants (mitochondria) work better. Lower doses didn’t help, and the benefit only showed when the peptide was given during the blockage, not after.

Utility 2
pubmed May 14, 2019

A possible role for mitochondrial-derived peptides humanin and MOTS-c in patients with Q fever fatigue syndrome and chronic fatigue syndrome.

Raijmakers. Ruud P H RPH; Jansen. Anne F M AFM; Keijmel. Stephan P SP; Ter Horst. Rob R; Roerink. Me...

The study found that people with Q fever fatigue syndrome, chronic fatigue syndrome, and even those who had been exposed to Q fever but feel fine have lower levels of the mitochondrial peptides humanin and MOTS‑c in their blood. This suggests these tiny proteins might be part of why they feel constantly tired, but the research didn’t test any treatments.

Utility 2
pubmed Nov 23, 2018

Follicular fluid humanin concentration is related to ovarian reserve markers and clinical pregnancy after IVF-ICSI: a pilot study.

Rao. Meng M; Zhou. Fang F; Tang. Li L; Zeng. Zhengyan Z; Hu. Shifu S; Wang. Yingying Y; Ke. Dandan D...

The study shows that the tiny protein humanin is naturally made in the ovary and shows up in the fluid around eggs. Higher levels of humanin in that fluid are linked to better markers of ovarian health and a higher chance of getting pregnant after IVF, but the research is only observational and doesn’t test taking humanin as a supplement.