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Humanin

HN, S14G-Humanin

A mitochondrial-derived peptide that protects against apoptosis, oxidative stress, and has potential in neurodegenerative and metabolic diseases.

Quick Stats
Studies 491
Trials 100
Formula C119H204N34O32S2
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Utility 3
pubmed May 13, 2003

Two serine residues distinctly regulate the rescue function of Humanin, an inhibiting factor of Alzheimer's disease-related neurotoxicity: functional potentiation by isomerization and dimerization.

Terashita. Kenzo K; Hashimoto. Yuichi Y; Niikura. Takako T; Tajima. Hirohisa H; Yamagishi. Yohichi Y...

Scientists found that tiny changes to the 24‑amino‑acid peptide Humanin make it dramatically better at protecting brain cells from Alzheimer‑related damage. Swapping the natural Serine at position 14 for its mirror‑image form (D‑Ser) boosts its protective power by 100‑ to 1,000‑fold, while the Serine at position 7 is needed for the peptide to pair up (dimerize), which is also essential for its effect. The most potent version worked at picomolar levels in lab tests, suggesting a path to ultra‑effective Humanin‑based therapies, though it’s still far from a usable supplement or drug.

Utility 3
pubmed Jun 6, 2019

Humanin prevents undesired apoptosis of chondrocytes without interfering with the anti-inflammatory effect of dexamethasone in collagen-induced arthritis.

Celvin. Bettina B; Zaman. Farasat F; Aulin. Cecilia C; Sävendahl. Lars L

In a mouse arthritis model, adding a synthetic humanin peptide (HNG) to dexamethasone stopped the drug from killing cartilage cells while still letting dexamethasone reduce joint inflammation. This means the peptide might protect bone growth when steroids are used long‑term, but the evidence is still only in animals.

Utility 3
pubmed Apr 25, 2010

Humanin protects neurons against apoptosis induced by Abeta31-35 through suppression of intrinsic pathway.

Li. Ling-Min LM; Zhang. Yu Y; Qiao. Jian-Tian JT; Zhang. Ce C

The study shows that the tiny protein humanin can protect brain cells in a dish from dying when they’re exposed to a harmful piece of amyloid‑beta, which is linked to Alzheimer’s. By giving the cells humanin before the toxin, the researchers saw less cell death, lower activity of death‑related enzymes, and blocked a key step that moves a death‑trigger protein into the mitochondria.

Utility 3
pubmed 2010

Mitochondrial dysfunction, proteotoxicity, and aging: causes or effects, and the possible impact of NAD+-controlled protein glycation.

Hipkiss. Alan R AR

The paper says that low NAD+ levels can cause harmful sugar‑derived chemicals that damage proteins and boost oxidative stress, while healthy mitochondria keep NAD+ high and help clear bad proteins. It also notes that making more mitochondria (through exercise, cold, etc.) raises protective molecules like humanin, which may aid aging and performance.

Utility 3
pubmed 2002

["Death and survival of neuronal cells exposed to Alzheimer's disease-relevant insults"].

Nishimoto. Ikuo I

Scientists found that a tiny protein called humanin (HN) can protect brain cells from the damage caused by Alzheimer’s‑related genes and toxic proteins. It works from outside the cell, and a slightly altered version (S14G‑HN) is about a thousand times more powerful. Humanin is normally made in the testis and colon, but shows up in the brains of people with Alzheimer’s, suggesting the body may try to use it as a defense.

Utility 2
pubmed Mar 22, 2024

Humanin variant P3S is associated with longevity in APOE4 carriers and resists APOE4-induced brain pathology.

Miller. Brendan B; Kim. Su-Jeong SJ; Cao. Kevin K; Mehta. Hemal H HH; Thumaty. Neehar N; Kumagai. Hi...

A special form of the tiny protein humanin, called P3S, is found more often in very old people who carry the APOE4 gene, which usually raises Alzheimer risk. In mice that mimic APOE4‑related brain disease, giving this P3S version lowered harmful amyloid‑beta buildup and boosted the brain’s ability to clear it away. Human brain data also showed that higher humanin levels link to genes that help clean up amyloid. While promising, the research is still early and done in animals, not humans.

Utility 2
pubmed Jan 21, 2025

Humanin reduces nucleus pulposus cells ferroptosis to alleviate intervertebral disc degeneration: An in vitro and in vivo study.

Zhu. Daxue D; Wang. Zhaoheng Z; Li. Yanhu Y; Chen. Shijie S; Kang. Xuewen X

Researchers found that the tiny protein humanin, which the body makes in mitochondria, is lower in people with back‑disc damage. Adding extra humanin in cell cultures and rats boosted the body’s own humanin, protected disc cells from a type of cell death called ferroptosis, and improved disc health in rats. The work shows humanin could one day be a treatment for disc degeneration, but it’s still early‑stage animal research.

Utility 2
pubmed Jan 16, 2024

Impact of Radiation Therapy on Serum Humanin and MOTS-c Levels in Patients with Lung or Breast Cancer.

Kavak. Ayse Gülbin AG; Karslioglu. Ihsan I; Saracaloglu. Ahmet A; Demiryürek. Seniz S; Dem...

The study measured two tiny proteins, humanin and MOTS‑c, in the blood of lung and breast cancer patients before and after radiation. Lung cancer patients naturally had higher MOTS‑c, and radiation made it rise even more. In breast cancer patients, radiation caused humanin levels to drop, while MOTS‑c stayed the same. The research shows these proteins respond differently to radiation depending on cancer type, but it doesn’t test any treatments or give clear advice for everyday health use.

Utility 2
pubmed Jan 8, 2025

Humanin alone and in combination with GnRHa therapy attenuates ovarian dysfunction induced by prepubertal cyclophosphamide chemotherapy in female mice.

Liu. Liu L; Wang. Huawei H; Wen. Wen W; Wang. Shunqing S; Zuo. Liqin L; Cheng. Yulin Y; Rao. Meng M;...

In a mouse study, a tiny protein called humanin helped protect young female mice’s ovaries from damage caused by the chemotherapy drug cyclophosphamide. The protection was seen in better follicle counts, hormone levels, and less oxidative stress, and adding a standard fertility drug (GnRHa) didn’t improve the effect further.

Utility 2
pubmed Nov 27, 2023

Effects of Humanin G (HNG) on angiogenesis and neurodegeneration markers in Age-related Macular Degeneration (AMD).

Nashine. Sonali S; Kenney. M Cristina MC

Humanin G, a tiny protein made by mitochondria, was tested on eye cells that mimic age‑related macular degeneration. In the lab it lowered cell death, helped the cells grow, and brought back normal levels of proteins linked to blood‑vessel growth and nerve damage. This suggests Humanin G could protect retinal cells, but the work was done only in petri dishes, not in people.

Utility 2
pubmed Jan 25, 2024

Humanin Treatment Protects Against Venetoclax-Induced Bone Growth Retardation in <i>Ex Vivo</i> Cultured Rat Bones.

Velentza. Lilly L; Wickstr&#xf6;m. Malin M; Kogner. Per P; Ohlsson. Claes C; Zaman. Farasat F; S&#xe...

In lab tests, the mitochondrial peptide humanin (as the HNG version) stopped the cancer drug venetoclax from slowing bone growth in rat bone cultures and human growth‑plate samples, but a single dose didn’t rescue bone growth in a short‑term mouse study, so the effect isn’t proven in living animals yet.

Utility 2
pubmed Nov 9, 2025

Insights into the Biomarker Potential of Humanin and Mots-c Expression and Telomere Length in Alzheimer's Disease.

Rodr&#xed;guez-Esparrag&#xf3;n. Francisco F; Cazorla-Rivero. Sara E SE; Torrealba. Eduardo E; C&#xe1...

The study found that the genetic messages (mRNA) for the mitochondrial peptides humanin and MOTS‑c are lower in people with Alzheimer’s disease, while the actual protein levels in blood don’t change much. This suggests the mRNA could be an early warning sign, but the research doesn’t give a clear way to use this information in everyday health hacks.

Utility 2
pubmed Nov 8, 2023

S14G-humanin confers cardioprotective effects against chronic adrenergic and pressure overload-induced heart failure in mice.

Zhao. Qi Q; Cai. Ming-Ming MM; Li. Dan D; Zhao. Bin-Yi BY; Zhou. Shuang-Shan SS; Wu. Zhen-Ru ZR; Shi...

A special form of the naturally‑occurring peptide humanin (called HNG) helped protect mouse hearts from damage caused by stress and high blood pressure. In two different mouse models of heart failure, giving HNG slowed down the decline in heart function, reduced scar tissue, inflammation, and cell death, and seemed to work through a signaling pathway called TGF‑beta. These results are promising but are still early‑stage animal research.

Utility 2
pubmed Nov 15, 2024

Humanin-G Ameliorates Hemorrhage-Induced Acute Lung Injury in Mice Through AMPK&#x3b1;1-Dependent and -Independent Mechanisms.

Amman. Allison M AM; Wolfe. Vivian V; Piraino. Giovanna G; Ziady. Assem A; Zingarelli. Basilia B

In a mouse study, a modified version of the mitochondrial peptide humanin (humanin‑G) helped protect lungs after severe blood loss. It reduced tissue damage and improved blood pressure, but its ability to lower immune cell buildup in the lungs depended on a specific protein (AMPKα1). The findings suggest humanin can boost certain protective pathways, though it’s still early‑stage and only tested in animals.

Utility 2
pubmed Apr 28, 2023

Effectiveness of analog of Humanin in ameliorating streptozotocin-induced diabetic nephropathy in Sprague Dawley rats.

Moin. Hira H; Shafi. Riffat R; Ishtiaq. Ayesha A; Liaquat. Afrose A; Majeed. Sadaf S; Zaidi. Nilofar...

In a rat study, a modified version of the mitochondrial peptide humanin (called S14G‑humanin) was given daily for 16 weeks and it helped reverse many signs of diabetic kidney damage, including high blood sugar, kidney waste markers, and inflammation. However, the work was done in animals, using injections that aren’t practical for people, and the exact dose for humans is unknown, so it’s more of a promising clue than a ready‑to‑use protocol.

Utility 2
pubmed Jul 14, 2023

S14G-Humanin ameliorates ovalbumin-induced airway inflammation in asthma mediated by inhibition of toll-like receptor 4 (TLR4) expression and the nuclear factor &#x3ba;-B (NF-&#x3ba;B)/early growth response protein-1 (Egr-1) pathway.

Su. Bo B; Li. Ran R; Song. Fuxing F; Liu. Min M; Sun. Xianjun X

In a mouse study, a more potent form of the peptide humanin called S14G‑humanin (HNG) reduced airway inflammation caused by an asthma trigger. Giving mice 2.5‑5 mg/kg of HNG lowered lung swelling, immune cell buildup, and inflammatory signals, and it also improved antioxidant levels in the lungs.

Utility 2
pubmed Jan 14, 2025

Plasma Humanin and Non-Coding RNAs as Biomarkers of Endothelial Dysfunction in Rheumatoid Arthritis: A Pilot Study.

Coradduzza. Donatella D; Cruciani. Sara S; Di Lorenzo. Biagio B; De Miglio. Maria Rosaria MR; Zinell...

In people with rheumatoid arthritis, having more of the naturally occurring peptide humanin in the blood was linked to healthier blood vessel lining and better survival, while certain RNA molecules didn’t show a connection. This suggests humanin might protect the cardiovascular system, but the study doesn’t tell us how to raise its levels or use it as a treatment yet.

Utility 2
pubmed Feb 1, 2025

Potential Role of High-Intensity Interval Training-Induced Increase in Humanin Levels for the Management of Type 2 Diabetes.

Soltany. Afsaneh A; Daryanoosh. Farhad F; Gholampour. Firouzeh F; Sadat Hosseini. Najmeh N; Khoramip...

In a rat study, 8 weeks of high‑intensity interval training (HIIT) raised the blood level of the peptide humanin and improved several markers of insulin resistance, inflammation, and oxidative stress in diabetic animals. The rise in humanin was linked to lower blood sugar and better muscle health, suggesting HIIT might help manage type‑2 diabetes partly through humanin. However, the research was done in rats, not people, so the findings are suggestive rather than definitive for humans.