Santana-Rodríguez. Norberto N; Llontop. Pedro P; Clavo. Bernardino B; Fiuza-Pérez. Mar...
No satisfactory treatment exists for chronic rejection (CR) after lung transplantation (LT). Our objective was to assess whether ozone (O<sub>3</sub>) treatment could ameliorate CR.
Male Sprague-Dawley inbred rats (n = 36) were randomly assigned into four groups: (1) control (n = 6), (2) sham (n = 6), (3) LT (n = 12), and (4) O<sub>3</sub>-LT (n = 12). Animals underwent left LT. O<sub>3</sub> was rectally administered daily for 2 weeks before LT (from 20 to 50 μg) and 3 times/wk (50 μg/dose) up to 3 months. CR; acute rejection; and Hspb27, Prdx, Epas1, Gpx3, Vegfa, Sftpa1, Sftpb, Plvap, Klf2, Cldn5, Thbd, Dsip, Fmo2, and Sepp1 mRNA gene expression were determined.
Severe CR was observed in all animals of LT group, but none of the O<sub>3</sub>-LT animals showed signs of CR, just a mild acute rejection was observed in 1 animal. A significant decrease of Hspb27, Prdx, Epas1, Gpx3, Vegfa, Sftpa1, Sftpb, Plvap, Klf2, Cldn5, Thbd, Dsip, and Fmo2 gene expression in the O<sub>3</sub>-LT group was observed CONCLUSIONS: O<sub>3</sub> therapy significantly delayed the onset of CR regulating the expression of genes involved in its pathogenesis. No known immunosuppressive therapy has been capable of achieving similar results. From a translational point of view, O<sub>3</sub> therapy could become a new adjuvant treatment for CR in patients undergoing LT.
Stanojlović. Olivera P OP; Zivanović. Dragana P DP; Mirković. Slobodan D SD; Mikha...
Previous studies have shown that humoral, endogenous and somnogenic, delta sleep-inducing peptide (DSIP) has influence on insomnia, pain, adaptation to stress, epilepsy, etc. We investigated the potential of DSIP and its analogue DSIP-12 (a nonapeptide with alanine in position 2 of DSIP molecule substituted by beta-alanine) to antagonize metaphit (1-[1(3-isothiocyanatophenyl)-cyclohexyl]piperidine) induced generalized, reflex audiogenic seizures in adult male Wistar albino rats.
The rats divided in four groups received (i.p.): saline; metaphit; metaphit+DSIP; and metaphit+DSIP-12, respectively. Metaphit-treated animals displaying seizure in eight previous tests received DSIP or DSIP-12 and afterwards audiogenic stimuli were applied at hourly intervals for the next 30 h. The animals were exposed to sound stimulation 60 min after metaphit administration and further on at hourly intervals. Incidence and severity of seizures were behaviorally analyzed. Selected EEGs and power spectra were recorded and analyzed.
Metaphit led to hypersynchronous epileptiform activity (polyspikes and spike-wave complexes) and increased power spectra 0.5-30 h after the treatment. Severity of metaphit seizures increased with time to reach the peak 7-12 h after injection. DSIP and DSIP-12 significantly (*P<0.05 and **P<0.01) increased in delta and theta frequency bands and decreased the incidence, mean seizure grade and duration of metaphit convulsions. The results suggest that DSIP and DSIP-12 may be considered as potential antiepileptics in the animal model, DSIP-12 being more efficient than DSIP.
Zhao. Liujun L; Hong. Jinjiong J; Wandtke. Meghan E ME; Xu. Rongming R; Ma. Weihu W; Jiang. Weiyu W;...
Researchers measured the angles and sizes of spinal bone pieces from C6 to T2 to see if a special frontâside screw can be safely placed there. They found the screw works at the lower neck (C6âC7) and sometimes at T1, but itâs basically impossible to use this approach at T2.
Umriukhin. P E PE; Koplik. E V EV; Sudakov. K V KV
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Sabzehei. Mohammad Kazem MK; Waisi. Shadi S; Shokouhi. Maryam M; Tapak. Leili L
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Zhang. Xin-Guo XG; Wang. Wen-Na WN; Zhang. Chun-Sheng CS; Li. Kun K; Ma. Guo-Di GD; Li. Jian-Yong JY
Sleep is a natural part of every individual's life. Delta sleep-inducing peptide (DSIP) is a nonapeptide that could promote sleep through the induction of slow wave sleep. However, little is known about the pharmacological effect of DSIP on insomnia.
The main objective of this study was to analyze the pharmacological effect of DSIP on insomnia.
We designed a fusion protein containing N-terminal TAT-based transduction domain followed by human serum albumin and DSIP and designated this protein as PHD fusion protein. The PHD fusion protein were expressed in Pichia pastoris and purified. Mice were administered single subcutaneous injections three concentrations of PHD fusion protein (0.5, 1, 2 mg/kg), and the pharmacological activity of PHD fusion protein was studied using classic pentobarbitalinduced sleep test.
We expressed the PHD fusion protein in P. pastoris; furthermore, the PHD fused protein was purified to near homogeneity by DEAE Sepharose FF, Phenyl Sepharose HP and Blue Sepharose 6 FF. Our result showed that the increase of pentobarbital-induced hypnotic effect characterized by reducing sleep latency and prolonged sleep duration was observed for increasing concentrations of PHD fusion protein (P<0.05); moreover, different dose of PHD fusion protein could induce the mice to re-sleep in a dose-dependent manner, whereas higher doses of PHD fusion protein (1.0, 2.0 mg/kg) significantly increased the rate of sleep re-onset compared with the vehicle group of mice (P<0.05).
PHD fusion protein increased the hypnotic effects of pentobarbital by reducing sleep latency and prolonged sleep duration. The present study suggested PHD fusion protein could be a new drug candidate for insomnia.
Grigorchuk. O S OS
We studied the cerebral mechanisms of positive and negative emotions in rats with different behavior in open field, reflecting stress resistance and neuronal effects of delta-sleep-inducing peptide (DSIP). In 20 male Wistar rats 107 neurons of dorsal hippocampus (57 neurons in active in open field--prognostically resistant to emotional stress and 50 inpassive--prognostically predisposed rats) were registered after positive (lateral hypothalamus--LH) and negative (ventromedial hypothalamus--VMH) emotional centers electric stimulation. Hippocampal neurons in active rats were less sensitive to stimulation of LH and VMH compared with passive ones. DSIP microiontophoretic application before LH stimulation decreases neuronal responses in both active and passive animals. DSIP increases dorsal hippocampus neurons sensitivity to VMH stimulation in active rats and decreases in passive ones.
Sendín-Raña. Pablo P; González-Castaño. Francisco Javier FJ; Gómez-Cuba. Fe...
The study proposes a new algorithm that lets peerâtoâpeer communication systems (used in things like mobile sensor networks) decide how often they need to refresh their connections. By using information about delays between devices, the algorithm cuts down on extra signaling traffic, which can save battery life and keep the network reliable.
Stanojlović. Olivera O; Hrncić. Dragan D; Radosavljević. Tatjana T
The same neuropeptides regulate both cicle sleep-wake and excitability of the brain.
Literature data together with our results support the idea that delta sleep--inducing peptide and neuropeptide Y could represent one of the factors of the endogenous stabilization of brain excitability and potent antiepileptic in generalized metaphit-induced audiogenic convulsive activity. The same holds true for DSIP analogues.
Bogolepov. N N NN; Popova. E N EN; Koplik. E V EV; Krivitskaya. G N GN; Sudakov. K V KV
Wistar rats with different levels of resistance to emotional stress (ES) were subjected to stress and brain sections stained with Nissl cresyl violet were used for quantitative analysis of the structural organization of neurons in layer V of the sensorimotor cortex. Some animals received delta sleep-inducing peptide (DSIP) 1 h before stress. Control ES-susceptible rats, as compared with resistant rats, had lower levels of normochromic and moderately hypochromic neurons. Normochromic neurons were not seen after stress. Rats susceptible to ES showed particularly sharp decreases in moderately hypo- and hyperchromic neurons, along with increases in the proportions of extremely hypo- and hyperchromic neurons, ghost cells, and ischemically altered cells. After administration of DSIP before stress, ischemically altered cells were not seen in any group: the level of reduction of extremely hyperchromic neurons was smaller in ES-susceptible rats than in ES-resistant rats. It is suggested that brain hypoxia plays a particular role in disorganizing the cortex in conditions of ES, while DSIP has both antistress and antihypoxic properties.
Stanojlović. Olivera P OP; Zivanović. Dragana P DP
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Popovich. Irina G IG; Voitenkov. Boris O BO; Anisimov. Vladimir N VN; Ivanov. Vadim T VT; Mikhaleva....
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Onoka. Isaac I; Banyika. Andrew Toyi AT; Banerjee. Protibha Nath PN; Makangara. John J JJ; Dujourdy....
The paper reviews how lawâenforcement labs track the chemical âfingerprintsâ of seized methamphetamine to figure out where it was made and how it was produced, but it does not give any healthâor performanceârelated advice for biohackers.
Antonângelo. Leila L; Tuma. Taila T; Fabro. Alexandre A; Acencio. Milena M; Terra. Ricardo R; P...
This study looked at three proteins (Idâ1, Idâ2, Idâ3) in lung cancer tissue and found that higher levels of Idâ1 and Idâ3 are linked to worse survival and more aggressive disease, but it doesnât give any advice you can use for everyday health or performance.
Kutilin. D S DS; Bondarenko. T I TI; Kornienko. I V IV; Mikhaleva. I I II
Subcutaneous injections of exogenous delta sleep-inducing peptide in a dose of 100 μg/kg (monthly, 5-day courses) to rats of various age groups (2-24 months) were followed by an increase in the expression of genes for SOD 1 (Sod1) and glutathione peroxidase 1 (Gpx1) in the brain and nucleated blood cells. The expression of these genes was shown to decrease during physiological aging of the body.
Konorova. I L IL; Gannushkina. I V IV; Koplik. E V EV; Antelava. A L AL
In a rat study, the peptide Deltaran helped keep blood flowing in the brain after a stroke, especially in animals that were stressed beforehand. It cut death rates by about twoâthirds and improved blood supply to brain cells, mainly on the left side of the brain.
Makletsova. M G MG; Mikhaleva. I I II; Prudchenko. I A IA; Rikhireva. G T GT
For evaluation of the nature of adaptogenic properties of delta sleep-inducing peptide we studied the effect of this substance on macromolecule biosynthesis in the brain of rats and mice exposed to burn injury and psychoemotional stress, respectively. Anabolic activity of delta sleep-inducing peptide depended on the purpose of adaptation corresponding to the type of stress.
Ashley. James J; Sorrentino. Violet V; Lobb-Rabe. Meike M; Nagarkar-Jaiswal. Sonal S; Tan. Liming L;...
Scientists studied two proteins, DIPâα and Dpr10, in fruitâfly larvae and found they help motor neurons connect to the right muscles. When either protein is missing, specific nerve branches fail to form correctly.
Venkatasubramanian. Lalanti L; Guo. Zhenhao Z; Xu. Shuwa S; Tan. Liming L; Xiao. Qi Q; Nagarkar-Jais...
This study looks at how certain proteins (DIPâα and Dpr10) help fruitâfly leg motor neurons form the right branching patterns when they connect to muscles. Itâs a basic neuroscience discovery about fly development, not a humanâfocused health finding.
Umryukhin. P E PE; Anokhin. K V KV; Raevskii. K S KS
In a rat study, a drug that blocks NMDA receptors (dizocilpine) stopped a sleepârelated peptide (delta sleepâinducing peptide, DSIP) from lowering a brain activity marker (câfos) in a specific hypothalamic region. This shows DSIP works through NMDA pathways, but the work is purely basic science and doesnât give any practical advice for using DSIP in people.